**Publication: Wireless Sensor Network***Author: de Marco, G; le Pellec, A*

*Date published: March 1, 2010*

*Language: English*

*PMID: 102313*

*ISSN: 19453078*

*Journal code: WSNK*

1. Introduction

Imaging can be used to locate the brain areas involved in various forms of motor behavior, attention, vision or emotion, self-awareness and awareness of others, but brain network modeling probably remains the greatest challenge in the field of imaging data analysis [1]. Neuroimaging first allowed researchers to describe the cortical and subcortical activity of regionally segregated functional regions during a variety of experimental or cognitive tasks. More recently, functional integration studies have described how these functionally special-ized areas, i.e., areas whose activity is temporally modi-fied, interact within a highly distributed neural network. By using functional magnetic resonance imaging (fMRI), which has become the method most commonly used to investigate human brain functions and define neural populations as distributed local networks transiently, linked by large-scale reciprocal dynamic connections [2].

After defining the concept of functional and effective connectivity, various approaches to the identification and modeling of circuits into networks will be presented in order to more realistically define the dynamics of the central nervous system which underlies various cerebral functions. A distinction should be made between meth-ods that only consider correlations and ignore issues of causality and influence and methods that attempt to de-scribe or draw inferences concerning the direction of influence between regions. Methodological approaches to the study of connectivity using fMRI data may be broadly divided into those that are more data-driven and attempt to map connectivity in the whole brain and those that use prior knowledge or hypotheses-driven, limited to a restricted set of regions [3]. These two categories of analysis are described, as indicated below, as functional connectivity and effective connectivity, respectively [4-6]. Techniques in the first group that consider only correlations between regions include mapping using seed-voxel correlations. Techniques in the second group use more elaborate models and additional assumptions applied to calculate correlations or covariances to ad-dress questions about directional influences and include mapping based on structural equation modeling (SEM), multivariate autoregressive (MAR) modeling, dynamic causal modeling (DCM).

2. Functional and Effective Connectivity

The dichotomy between local and large-scale networks serves as a neural basis for the key assumption that brain functional architecture abides by two principles: func-tional segregation and functional integration [2,3,7]. A large-scale brain network can be defined as a set of seg-regated and integrated regions that share strong ana-tomical connections and functional interactions. Whether top-down or bottom-up, connections and interactions are quintessential aspects of networks [8,9]. Cognitive and sensorimotor processes depend on complex dynamics of temporally and spatially segregated brain activities. While the segregation principle states that some func-tional processes specifically engage well-localized and specialized brain regions, it is now thought that brain functions are most likely to emerge through integration of information flows across widely distributed regions [2,10,11]. According to this approach, it is not only iso-lated brain areas that are presumed to process information but rather a large-scale network, i.e. a set of brain regions interacting in a coherent and dynamic way. Hence, according to the functional integration concept, cortical areas and therefore functions are integrated within specific dynamic networks.

This concept supposes the existence of a dynamic in-teraction between interconnected, active areas and that the brain areas are expressed as networks within inte-grated systems. In such a system, localized areas are in-cluded in networks which become dynamic according to the cognitive task. Brain areas underlie several functions and can belong successively to several different func-tional networks. In other words, a given brain area does not have a single function; its resources can be exploited in several different cognitive strategies. The principle of functional integration which is also known in the field of electrophysiology was used to analyze the event poten-tials obtained from multielectrode recordings [12]. Thus, based on the functional integration principle, the rela-tionships between several brain areas may be examined.

Effective connectivity, closer to the intuitive notion of a connection, can be defined as the influence that one neural system exerts over another, either at a synaptic level (synaptic efficacy) or a cortical level [13,14]. This approach emphasizes that determining effective connec-tivity requires a causal model of the interactions between the elements of the neural system of interest. In electro-physiology, there is a close relationship between effec-tive connectivity and synaptic efficacy [15]. Effective connectivity can be estimated from linear models to test whether a theoretical model seeking to explain a network of relationships can actually fit the relationships esti-mated from the observed data. In the case of fMRI, the theoretical model is an anatomically constrained model and the data are interregional covariances of activity [16].

Consequently, effective connectivity represents the dynamic influence that cortical and subcortical regions exert on each other via a putative network of interde-pendent areas [5,12]. This approach might be based on linear time-invariant models that relate the time-course of experimentally controlled manipulations to BOLD signals in a voxel-specific fashion. Although various statistical models have been proposed [17], these stan-dard models treat the voxels throughout the brain as iso-lated black boxes, whose input-output functions are characterized by BOLD responses evoked by various experimental conditions [18]. fMRI provides simultane-ous recordings of activity throughout the brain evoked by cognitive and sensorimotor challenges, but at the ex-pense of ignoring temporal information, i.e., the history of the experimental task (input) or physiologic variable (signal). This is important, as interactions within the brain, whether over short or long distances, take time and are not instantaneous which is implicit within regression models. Furthermore, the instantaneous state of any brain system that conforms to a dynamic system will depend on the history of its input.

3. Data-Driven Approaches

The first category of methods includes seed-voxel corre-lations, Granger causality derived autoregressive models [19], fuzzy clustering which assumes that brain voxels can be grouped into clusters sharing similar activity pat-terns [20-22], hierarchical clustering [23,24], psycho-physiologic interactions which test for changes in the regression slope of activity at every voxel on a seed voxel that are induced by an experimental manipulation [25], and spectral analysis [26-28]. Other techniques, such as principal component analysis [29-31] and inde-pendent component analysis (ICA) [32-35], suppose that fMRI data are a linear mixing of a given number of temporal factors with an associated factor-specific spatial distribution. Among all of these methods, we propose to briefly describe the ICA method (time analysis of the BOLD response) and the spectral method (frequency analysis of the time response) that are two interesting methods to spatially identify circuits within networks in the brain.

3.1. Independent Component Analysis

Independent component analysis (ICA) is a data-based multivariate statistical technique that uses higher order statistics to perform decomposition of linearly combined statistically independent sources [36]. Each statistically independent component represents a hemodynamic map of the whole brain. Each independent component is sup-posed to describe a particular functional activity of the brain with its deployment over time [37-39]. Each inde-pendent component extracted by applying a spatial ICA is spatially independent of all other independent compo-nents [35]. Therefore, the contribution of a spatial inde-pendent component to each voxel is given by the inde-pendent component magnitude at that point modulated over time by the associated time-course. The main ad-vantage of ICA is that it requires little knowledge about the nature of the data. The only necessary hypothesis concerns the presence of a sufficient amount of inde-pendent sources (temporal or spatial), which are linearly mixed. Conversely, one of the main drawbacks of ICA is the large amount of brain activations resulting from this kind of decomposition [40]. At some point, hypotheses are necessary to select relevant from spurious activa-tions.

For this reason, ICA can be used in conjunction with other well-established techniques [41] or further infor-mation may be associated with the reference time-course, such as the spatial localization of activities [42] and the covariate relation of independent component time-course [43]. ICA could be combined with SEM to extend the explanatory power of each technique. SEM is a well de-veloped, computationally minimally intensive connec-tivity analysis technique suitable for neuroimaging data, especially when it is combined with other data-driven methods such as ICA. In this case, SEM coupled with ICA is capable to handle data from a large number of subjects [32]. The biological relevance and cortical con-nections of the SEM models have also been evaluated with reference to available knowledge based on animal and human circuitries. The main advantage of spatial group ICA is its ability to identify the distinct functional elements involved in the circuitry [33]. Functionally connected brain regions encompassed in each independ-ent component are active at the same time, suggesting that one or more anatomical connections are in use dur-ing performance of the task. Although this reasoning is more in line with the "connectionist" approach to brain functions based on parallel processing mechanisms per-formed by a group of connected functional elements, the ICA approach lacks a statistical method to model the functional connections assumed to exist between regions. The addition of ICA to SEM can address this issue. Each ICA map or part of the map corresponds to one compo-nent in an SEM.

3.2. Spectral Analysis

The description of a correlation structure in the fre-quency domain can be a promising approach to investi-gate interregional strengths of interactions of a functional network. As time-dependent correlations may vary be-tween fMRI signals and across the space independently of the underlying neural dynamics, a method of analysis of frequency-dependent correlations would be one way to overcome this interregional variability of the BOLD response and would also be crucial for extracting the fine detail of information hidden within the fMRI signal. Functional connectivity analysis in the presence of major physiologic noise sources is a pitfall especially when the correlation (or covariance) between BOLD signals is performed in the time domain. In this case, these noise sources may artificially increase the magnitude of cross-correlation. Estimation of coherence between pairs of voxels at a specific frequency or at a limited range of frequencies can therefore be one way to deal with nox-ious physiologic noise.

The frequency domain approach can be used to ana-lyze a limited range of linear relationships within a re-stricted frequency band [44]. Consequently, measure-ment of the correlation between fMRI data can be en-hanced and can help to resolve the problem of false con-nectivity derived from cardiac and respiratory cycles and/or vascular differences. This approach can be per-formed by using spectral analysis, which allows exami-nation of the structure of covariance and provides certain voxel-based parameters such as coherence which as-sesses the dependence between voxel signals [26].

The spectral theory for multivariate time series has al-ready been used in several fMRI studies [27,28,45]. By using fMRI signals, these authors demonstrated that time domain approaches may be sufficiently susceptible to substantially high frequency artefacts, whereas the spec-tral domain is essentially resistant to these artefacts. They also demonstrated that the frequency-dependent correlation is higher than that measured in the temporal domain. In other fields of neuroscience, for instance in electroencephalography (EEG), coherence analysis is widely used to investigate correlated oscillatory activities between various areas in the brain [46-49]. In magne-toencephalography (MEG), coherence analysis has also been demonstrated to be a useful technique in clinical studies for discriminating different rhythmic behaviors in various brain regions [50-52]. However, although the relationship between neuronal currents and hemody-namic response is poorly understood, simultaneous in-tracortical neural recordings and fMRI signals acquired in animals recently revealed a significant correlation between local field potential and vascular response [53]. The feasibility of a correlation between the synchrony of low frequency BOLD fluctuations in functionally related brain regions and neuronal connections that facilitate coordinated activities has been demonstrated in various applications [54,55].

4. Hypothesis-Driven Approaches

The alternative to data-based approaches is to use a model that attempts to describe the relationships between a set of selected regions, in which region-specific meas-urements such as BOLD time series are extracted from whole-brain data prior to the connectivity modeling stage. This category includes structural equation modeling (SEM) [56-62], multivariate autoregressive (MAR) modeling [63,64], dynamic causal modeling (DCM) [65-67], generative models including neural mass mod-els [68,69] and large-scale neural models [70-72].

4.1. Structural Equation Modelling

Path analysis, also referred to as structural equation modeling (SEM), was originally developed in the early 1970s by Jöreskog, Keesling, and Wiley, when they combined factor analysis with econometric simultaneous equation models [73-76]. In the early 1990s, McIntosh introduced SEM to neuroimaging [56,59,77-79] for modeling, testing, and comparison of directional effec-tive connectivity of the brain. SEM rapidly became popular in this field [31,57,80-86]. Structural models can be used to analyze linear relationships between variables from analysis of the covariance among the variables. Structural models were developed from two principal methods of analyses: factorial analysis (for a review: [75]) and multiple regression or causal path analysis (a method developed in the 1930s by Wright e.g., (for a review: [87]). Structural models examine multiple sources of influence on the dependent variable in an ex-periment [88,89].

Structural Equation Modeling (SEM) is a hypothe-sis-based multivariate statistical technique of data analy-sis that can be used with neuroimaging data. An increas-ing number of PET, fMRI and transcranial magnetic stimulation (TMS) studies have used SEM to investigate large-scale functional brain networks [90-93] and show specific networks involved in either working memory [94-100], attentional processes [64,101-103], face per-ception [104-106], motor movement processing [61, 107-112], language [32,113,114] or processing of pain-ful stimuli [62].

SEM methods, in comparison with classical ap-proaches such as linear regression, allow simultaneous analysis of several types of interrelationships between variables in an experiment [13,115-117]. The nature of the relationship between variables is given by the regres-sion coefficient; it describes how much the dependent variable changes when an independent variable changes by one unit. SEM directly integrates measurement errors into a statistical model, so that estimates of regression coefficients are consequently more precise than with classical methods such as multiple regression, factorial analysis, or analysis of variance. The older methods ex-amine only one linear relationship at the same time be-tween independent and dependant variables and only within a range of values set by the investigator [14]. In contrast with classical methods, SEM analyzes a struc-ture of variances and covariances in a dataset of observed variables and can be used to predict dependences be-tween variables. In other words, SEM seeks to explain as much of the variance in dependant variables as it can from simultaneous measurement of the variances of the independent variables included in the model. Similarly, SEM incorporates measurement errors of the independ-ent variables into calculation of the estimate, which re-inforces the statistical power of the method and provides more precise estimates of regression coefficients. A model of measurement can therefore be validated from a theoretical model or empirical data [99]. The objective of effective connectivity analysis is to estimate parameters that represent influences between regions that may change over time and with respect to experimental tasks.

In order to describe a functional network, network nodes and anatomical connections must therefore be proposed in conjunction with a SEM model to explain interregional covariances and determine the intensity of the connections. When applied to PET or fMRI data, SEM allows modeling of connection pathways between cortical or subcortical areas and reveals relationships, interdependencies and covariance between the various areas. In a given anatomical model, SEM shows the ef-fects of an experimental task on a specific network of connections [14,118-120]. In this type of statistical analysis, normalized variables are considered in terms of the structure of their covariances. SEM therefore allows inference of interregional dependencies between various cerebral cortical areas.

SEM is a simple and pragmatic approach to effective connectivity when dynamic aspects can be disregarded. A linear model is sufficient and the observed variables can be measured precisely, the input is unknown but stochastic and stationary. SEM comprises a set of regions and a set of directed connections. Importantly, a causal relationship is ascribed to these connections. Causal rela-tionships are therefore not inferred from the data, but are assumed a priori. The strengths of connections can therefore be set so as to minimize the discrepancy be-tween observed and implied correlations and thereby fit a model to the data. Changes in connectivity can be attrib-uted to experimental manipulation by partitioning the data set. If, for example, a given fMRI data set is parti-tioned into those scans obtained for different levels of an experimental factor, differences in connectivity can then be attributed to that factor leading to the conclusion that a pathway has been activated. An SEM with particular connection strengths implies a particular set of instanta-neous correlations between regions. Structural equation models posit a set of theoretical causal relationships be-tween variables and model instantaneous correlations i.e., correlations between regions at the same time-point. In-stantaneous activity is assumed to be the result of local dynamics and connections between regions.

4.2. Multivariate Autoregressive (MAR) Models

To overcome the difficulties of SEM, Harrison et al. pro-posed the use of multivariate autoregressive (MAR) models for the analysis of fMRI data [63]. They were the first to introduce multivariate autoregressive (MAR) models into brain pathway analyses to characterize in-terregional dependence. MAR models are time-series models and consequently model temporal order within measured brain activity. Goebel et al. [19] and Roe-broeck et al. [121] subsequently generalized the MAR approach by incorporating Granger causality between two time series. MAR models posit a set of causal relation-ships between variables; they incorporate cross-covariances between regions (covariances at multiple lags) and ex-ploit temporal relationships between different scans to allow conclusions about predominant directions of in-fluence between regions as well as their strength [18, 122,123].

An autoregressive approach is used to characterize a structure in a time series, whereby the current value of a time series is modeled as a weighted linear sum of pre-vious values. Consecutive measurements within a given time series contain information about the process that generated this series. This is an autoregressive process and is a very simple, yet effective, approach to time se-ries characterization. This is distinct from regression techniques that quantify instantaneous correlations, but is similar to the SEM model in that it estimates the relative influences over time. Autoregressive models of fMRI data address the temporal aspect of causality in a BOLD time series, focusing on the causal dependence of the present on the past. Each data point of a time series is explained as a linear combination of past data points. This approach contrasts with SEM regression-based models in which the time series can be permuted without changing the results. MAR models contain directed in-fluences among a set of regions whose causal interac-tions, expressed at the BOLD level, are inferred via their mutual predictability from past time points.

4.3. Dynamic Causal Modeling

A major criticism of SEM or MAR with regard to neuroimaging data is that they model effective connec-tivity changes at the "hemodynamic level" rather than the "neuronal level". This is a serious problem because the causal architecture of the system that we want to identify is expressed in terms of neuronal dynamics, which are not directly observed using noninvasive tech-niques. In the case of fMRI data, previous models of effective connectivity have been fitted to the measured time series which result from a hemodynamic convolu-tion of the underlying neural activity. Since classical statistical models do not include the forward model link-ing neuronal activity to the measured hemodynamic data, analyses of interregional connectivity performed on hemodynamic responses are problematic. For example, different brain regions can exhibit marked differences in neurovascular coupling, and these differences, expressed in different latencies (see above) may lead to false infer-ences about connectivity [124].

Dynamical Causal Modeling (DCM) has recently been developed as a generalization of both convolution mod-els and SEM [66,67]. As described in Penny et al. [66], SEM can be shown to be a simplified version of DCM which also depends on the definition of a structural model. DCM model assumes a dynamic neuronal model of interacting brain regions, whereby neuronal activity in a given brain region causes changes in neuronal activity in other regions according to the structural model. This neuronal model is then supplemented with a forward model of how neuronal activity generates a measured BOLD response through the balloon model which was initially formulated by Buxton et al. [125] and later ex-tended by Friston et al. [126]. A Bayesian inference scheme is devised to infer the model parameters from the data. The mathematical framework of DCM takes into account nonlinearities and temporal correlations. It also quantifies the interaction strength that one brain region exerts on another brain region at the neuronal level, whereas SEM only concerns the observed BOLD signal. DCM is suspected to be less sensitive than SEM to the number of degrees of freedom. Unlike SEM, DCM also models the effect of experimental, external, and modula-tory inputs on network dynamics. Since DCM models neurobiologically plausible neural activities and takes into account dynamics and modulations, this mathemati-cal framework would appear to be more advantageous than SEM.

4.4. Diffusion Tensor Imaging

While fMRI provides detailed information about the spa-tial location of functionally active cortical areas, the question of anatomical interdependency between cortical areas remains elusive. A key tool to assess the validity of large-scale distributed networks in fMRI is knowledge of the underlying anatomical connections. The original idea behind SEM and functional neuroimaging was to com-bine two data sets: a functional set with an anatomical set (connections between regions), based on the assumption that anatomy was the source of spatial causal relation-ships. Our understanding of the connections between regions is limited, but since the advent of newer tracto-graphy methods, the main white matter tracts can be de-scribed. Diffusion Tensor Imaging (DTI) is a powerful MRI technique [127,128] that can be used to translate self-diffusion, or microscopic motion of water molecules in tissue into a MRI measure of tissue integrity and structure (white matter fibers). Data from diffusion ten-sor imaging (DTI) and fMRI have been combined in a few previous studies [129-131]. These studies showed that a combination of techniques can give additional in-formation about brain organization which may give more specific information about organization of brain func-tions and brain injuries. In this latter case, a DTI-driven SEM would integrate information about white matter changes (e.g. maturation, aging) [100,132]. The prospect of using information derived from tractography could be used to constrain structural models. DTI and fMRI com-binations will be essential to discover to what extent the brain functional organization as investigated with fMRI reflects structural features of the brain and, hence, to more accurately assess the relevance of fMRI to examine the relationship between functional and large-scale ana-tomical networks. However, more studies are still needed to investigate anatomical correlates which would be re- lated to effective connectivity.

5. Conclusions

This article describes the most recent imaging ap-proaches used to explore and identify circuits within networks and to spatially and anatomically model inter-connected regions. Structural equation modeling is the most widely used method to model effective connectivity [56,82,133]. The relevance of applying SEM to fMRI neuroimaging data has been discussed in detail elsewhere [58,66,82,134]. SEM allows one to start with simpler models and then progress to more complex models by repeatedly testing the model fit to real data. SEM is use-ful when some information is available, such as a small set of potential structural models or partial information concerning connectivity. Newer, more sophisticated ef-fective connectivity analysis methods such as Dynamic Causal Modeling might circumvent the drawbacks of SEM and may shed more insight into how brain regions interact in information processing. Nevertheless, SEM is a well developed, computationally less intensive connec-tivity analysis technique suitable for neuroimaging data especially for block designs and combined with other methods such as independent component analysis, partial correlation or DTI. The use of SEM may be justified by the fact that, unlike DCM, the statistical model underly-ing SEM is quite simple and not computationally de-manding.

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Author affiliation:

G. de Marco, A. le Pellec

Université Paris X, Laboratoire du contrôle moteur et d'analyse du mouvement, Paris, France

E-mail: demarco.giovanni@gmail.com

Received November 24, 2009; revised December 19, 2009; accepted January 8, 2010