MORPHOLOGICAL AND HISTOLOGICAL STUDIES OF CARDIOSPERMUM HALICACABUM L. EXPLANTS IN CALLUS CULTURE

Cardiospermum halicacabum L. is an important medicinal twining herb belonging to the family sapindaceae. Callus cultures were obtained from leaves, cotyledon, hypocotyl, epicotyl and radicle explants fromin vitro grown seedlings of Cardiospermum halicacabum L. inoculated on MS and B5 media containing various concentrations of 2,4- D (0.5 - 2.5mg/l) and BAP (0.5 - 2.5 mg/l). The media and growth regulators stimulated various types of calli. Pale yellow and brown coloured friable callus was obtained on MS and B5 media supplemented with various concentrations of 2, 4 - D from radicle explants; whereas hypocotyl and epicotyl showed pale yellow and cream coloured good amount of compact and friable callus respectively. The leaf explant demonstrated direct rhizogenesis on B5 medium fortified with BAP (0.5 - 2.5 mg/l) whereas hypocotyl showed compact green coloured callus. The histological studies gave an idea of xylem elements and nodules of meristemoids in callus sections. Iodine staining exhibited a strong starch accumulation in callus tissues and in prenodular structures. Key words: Cardiospermumhalicacabum L., Callus culture.

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