Author: Singh, R K; Mittal, P K; Gourshettiwar, M P; Pande, S J; Dhiman, R C
Date published: March 1, 2012
Malaria is hyperendemic in central parts of India with 95% of the population at risk of infection1. In Maharashtra state, Gadchiroli district is one of the high malaria endemic districts where 13,553 malaria positive cases were reported in the year 2010, out of which Plasmodium falciparum accounted for 82.4%2. Slide positivity rate (SPR) in Gadchiroli district has been ranging from as low as 1.43 to as high as 2.5% between the years 2006 and 20102. But it has been substantially higher (2.5%) in 2010.
Geographical area of Gadchiroli district is 14,412 km2 and it is located on the north-eastern side of the state of Maharashtra. It is situated at 18.43' to 21.50' north latitude and 79.45' to 80.53' east longitude and has uneven terrain with hills, valleys and forests at different altitudes and having total population of 10,71,795 (as of census 2011)3. The average rainfall is 1743.5 mm, temperature minimum 11.3 and maximum 47.7oC.
Since 1958, the district has been receiving two rounds of sprays of DDT every year. An. culicifacies has developed resistance to DDT, dieldrin and malathion (triple resistance) in about 30 districts of Maharashtra. Presently, this area is receiving two rounds of spray with a synthetic pyrethroid, alpha-cypermethrin. In spite of these control measures, malaria remains a major public health problem. The primary vectors of malaria in Gadchiroli district are An. culicifacies and An. fluviatilis4,5.
In India, An. culicifacies Giles alone contributes about 65% of the total cases of malaria annually and is the widespread malaria vector of rural and peri-urban areas. However, this species has developed resistance to DDT in 286 districts, to malathion in 81 and to pyrethroids in 2 districts in India6-8. In order to find out the rationale about using alpha-cypermethrin in vector control, this study was carried out on insecticide susceptibility in 10 endemic villages of PHC, Murumgaon, Gadchiroli district of Maharashtra state.
Insecticides susceptibility tests were carried out in the the year 2010 by using WHO procedure against various insecticides9-10. In the morning hours (0600 to 0800 hrs) with the help of suction tube, An. culicifacies Giles, An. fluviatilis James and An. annularis Van der Wulp were collected from cattlesheds, human dwellings and mixed dwellings of different villages of Murumgaon PHC. The collected female mosquitoes were provided with 10% glucose solution soaked in cotton pads and transported in caged cloth to the field laboratories at the PHC of Murumgaon, Gadchiroli district. Insecticide-impregnated papers with different diagnostic doses received from University Sans Malaysia were used for detection of resistance to DDT (4%), malathion (5%) and deltamethrin (0.05%).
Only full-fed and semi-gravid mosquitoes were used to determine the susceptibility status. Mosquitoes were exposed against the diagnostic doses of insecticides for an hour, as per standard WHO technique9,10. Three to four replicates of each vector species containing 15-25 female mosquitoes were taken simultaneously for each insecticide. Two replicates for control were also held parallel to each test. The mosquitoes were exposed for 1 h and kept for observation for 24 h.The percent mortalities were scored after 24 h of recovery period and if the control mortality remained within 5-20%, test mortality was corrected using Abbott's formula11 and expressed as corrected percent mortality. If the mortality is >20%, the tests were discarded.
Results of susceptibility tests on An. culicifacies, An. fluviatilis and An. annularis against DDT (4%), malathion (5%) and deltamethrin (0.05%) are given in Table 1. The corrected percent mortality of adult An. culicifacies, An. fluviatilis and An. annularis to DDT (4%) ranged between 21 and 36.66%; to malathion (5%) between 90.52 and 96%; and to deltamethrin (0.05%) between 94 and 96.42%. These results indicate that the tested mosquito species are resistant to DDT but tolerant to malathion and deltamethrin.
In India, resistance in malaria vectors to DDT at sev eral places has been reported6-8. First indication of resistance to DDT in An. culicifacies malaria vectors was found in Maharashtra state by Rao and Bhatia12 in the year 1957. However, first confirmed report on DDT resistance was published by Rahman et al13 in 1959. Later, Krishnamurthy and Singh14 recorded resistance in An. culicifacies to DDT in a village of Uttar Pradesh in the year 1962. Double or triple resistance to DDT, dieldrin and malathion was recorded in An. culicifacies from 30 districts of Maharashtra12,15-18. Resistance in An. culicifacies to malathion was first time observed from the adjoining state of Gujarat by Rajagopal et al19 in the year 1977. Raghavendra et al20 in the year 1991 reported malathion resistance in adjoining state of Andhra Pradesh. Dhiman et al21 in the year 2005 reported resistance to DDT in An. culicifacies from Dhanora taluka in Gadchiroli district, but it was found susceptible to other insecticides. Our study confirmed this finding in Murumgaon PHC area of District Gadchiroli, where An. culicifacies, as well as An. fluviatilis and An. annularis were found resistant to DDT only, while tolerant to other insecticides tested.
Anopheles fluviatilis has been reported as a primary vector of malaria in the forests of adjoining States of Chhattisgarh including Maharashtra4,5. Anopheles fluviatilis is found to transmit malaria throughout the year in these areas, whereas An. culicifacies transmit malaria in post-monsoon months between July and September. Anopheles fluviatilis is mainly distributed in hilly tract villages and the preferential breeding habitat is stream22,23, where the chances of exposure to the agriculture pesticides are relatively less. Anopheles fluviatilis is resistance to DDT in 11 districts from 8 states including Maharashtra7. Resistance to DDT in adult An. fluviatilis has also been reported from Orissa (now Odisha)24. However, Sharma et al25 reported that An. fluviatilis was found susceptible to DDT, malathion and deltamethrin in seven districts of Odisha. Singh et al26 recently reported resistance in An. culicifacies, An. fluviatilis and An. annularis to DDT from Jharkhand in the year 201027. In our study, An. fluviatilis was found resistant to DDT only, while they are tolerant to other insecticides tested. Resistance to DDT in An. fluviatilis may be due to prolonged use of DDT in indoor residual spray since 1958. However, presently this area is receiving two rounds of IRS with synthetic pyrethroids to which these species are in tolerant stages (verification required).
For the first time, An. annularis resistance to DDT was detected in the year1962 from Meerut district of Uttar Pradesh14. Later on resistance was reported from various parts of the country6-8, 24-28. Anopheles annularis has developed double resistance to DDT and dieldrin from Assam7. In our study, An. annularis was found resistant to DDT only, while they are at verification required stage to malathion and deltamethrin.
Present study indicated that An. culicifacies, An. fluviatilis and An. annularis are resistant to DDT while tolerant in case of other insecticides tested. Malathion resistance has been reported in An. culicifacies and An. annularis in earlier studies in Maharashtra, but our study showed no indication of malathion resistance in any of the tested mosquitoes species in this study area. High endemicity of malaria in the study area, which is receiving two rounds of IRS with synthetic pyrethroids, warrants that synthetic pyrethroids use should be undertaken as per norms of the National Vector Borne Disease Control Programme, where, there is a need to educate community for enhanced spray coverage of houses and rooms. There is also need to strictly monitor whether two rounds of IRS activities are done during coverage of houses. To delay the precipitation of resistance to synthetic pyrethroid, other insecticides can be used in rotation with synthetic pyrethroids. District-wise susceptibility status of mosquitoes to the insecticides being used in the programme should be monitored for judicious use of appropriate insecticides for malaria control in these areas.
The laboratory and field assistance provided by the staff of NIMR and Mr. Surendra Kumar is gratefully acknowledged. Authors are also thankful to Mr. Mather Boina, ADMO and his staff for providing the information on malaria incidence and use of insecticide in IRS in vector control in Gadchiroli district of Maharashtra state.
1. Delhi, India: National Vector Borne Disease Control Programme, Ministry of Health and Family Welfare. Available from: http:// www.nvbdcp.gov.in/malaria (accessed on June 20, 2011).
2. Government of Maharastra Public Health Department, Directorate of Health Services. Available from: http://mahaarogya. gov.in/programs/nhp/antimaleria/perfdistwise.htm
3. Census 2011. Available from: http:/www.census2011.co.in/census/ district/346-gadchirolil.htm.
4. Sharma MD. Vectors of malaria An. fluviatilis James 1902- vectors of malaria in India. Nat Soc Bull Ind Mal Mosq Dis 1961; 77-90.
5. Rao TR. The Anopheline of India 1984. Delhi: Malaria Research Centre (ICMR) 1984; p. 154-8.
6. Pillai, MKK. Vector resistance to insecticides. Proc Nat Acad Sci 1996; 68(B): 77-97.
7. Kumari R, Thapar BR, Dasgupta RK, Kaul SM, Lal S. Susceptibility status of malaria vectors to insecticides in India. J Commun Dis 1998; 30(3): 179-85.
8. Mittal PK, Wijeyratne P, Pandey S. Status of insecticide resistance of malaria, kala-azar, Japanese encephalitis vectors in Bangladesh, Bhutan, India and Nepal (BBIN). Available from: http//:ehpproj.org/pdf/Activity report 129. EHP Project 26568/ EXANEMDR.
9. Manual on practical entomology in malaria. Pt. II. Methods and techniques. Geneva: World Health Organization 1975; p. 191.
10. Instructions for determining the susceptibility or resistance of adult mosquito to organochlorine, organophosphate and carbonate insecticides - diagnostic test. WHO/VBC/1981, 81-806, p. 7.
11. Abbott WS. A method of computing the effectiveness of an insecticide. J Ecol Entomol 1925; 18: 265-467.
12. Rao TR, Bhatia SC. A note on the degree of susceptibility of Anopheles culicifacies to DDT in some parts of Bombay state, India. Indian J Malariol 1957; 11(3): 261-9.
13. Rahman J, Roy ML, Singh NN. Development of increased tolerance to DDT in Anopheles culicifacies Giles in the Panchmahal district in Bombay state, India. Indian J Malariol 1959; 12: 125-30.
14. Krishnamurthy BS, Singh NN. DDT resistance in Anopheles culicifacies Giles, 1901 and Anopheles annularis Van der Wulp 1884 in a village of Meerut district, U.P. Indian J Malariol 1962; 16: 375-7.
15. Vittal M, Bhate MR. Bioassay tests on the effectiveness of malathion spraying on Anopheles culicifacies resting on different wall surfaces in Aurangabad town, Maharashtra. Indian J Malariol 1981; 18: 124-5.
16. Vittal M, Deshpande LB. Development of malathion resistance in a DDT, HCH resistant Anopheles culicifacies population in Thane district (Maharashtra). J Commun Dis 1983; 15: 144-5.
17. Deobhankar RK, Pelkar ND. Magnitude of DDT resistance in Anopheles culicifacies in Maharashtra state. J Commun Dis 1990; 22: 77.
18. Sharma MID, Samnotra KG. A note on gamma BHC and dieldren resistance in Anopheles culicifacies Giles in adjoining areas of Gujarat and Maharashtra states. Bull Natl Soc India Malar Mosq Dis 1962; 10: 151-7.
19. Rajagopal R. Malathion resistance in Anopheles culicifacies in Gujarat. Indian J Med Res 1977; 66: 27-8.
20. Raghavendra K, Vasantha K, Subbarao SK, Pillai MKK, Sharma VP. Resistance in Anopheles culicifacies sibling species B and C to malathion in Andhra Pradesh and Gujarat states in India. J Amer Mosq Control Assoc 1991; 7(2): 255-9.
21. Dhiman RC, B Shahi, Sharma SN, Khargiwarkar VN, Subbarao SK. Persistence of malaria transmission in a tribal area in Maharashtra. Curr Sci 2005; 88(3): 754-8.
22. Singh N, Khare KK. Forest malaria in Madhya Pradesh: changing scenario of disease and its vectors. Indian J Parasit Dis 1999; 23: 105-12.
23. Das PK, Gunasekaran K, Sahu SS, Sadanandane C, Jambulingam P. Seasonal prevalence and resting behaviour of malaria vectors in Koraput district of Orissa. Indian J Malariol 1990; 27: 173-81.
24. Sahu SS, Patra KP. A study on insecticides resistance in Anopheles fluviatilis and An. culicifacies to HCH and DDT in Malkangiri district of Orissa. Indian J Malariol 1995; 32(3): 112-8.
25. Sharma SK, Upadhyay AK, Haque MA, Singh OP, Adak T, Subbarao SK. Insecticide susceptibility status of malaria vectors in some hyper-endemic tribal districts of Orissa. Curr Sci 2004; 87(12): 1718-26.
26. Singh RK, Dhiman RC, Mittal PK, Das MK. Susceptibility of malaria vectors to insecticides in Gumla district, Jharkhand state, India. J Vector Borne Dis 2010; 47: 116-8.
27. Azeez SA. Susceptibility status of Anopheles fluviatilis and Anopheles annularis to DDT in an area near Dhanbad. Bull Indian Soc Mal Commun Dis 1964; 1: 53-4.
28. Singh RK, Dhiman RC, Kumar G, Sinha ATS, Dua VK. Susceptibility of malaria vectors to insecticides in Koderma, Jharkhand. J Commun Dis 2011; 43: 273-6.
R.K. Singh1, P.K. Mittal1, M.P. Gourshettiwar2, S.J. Pande3 & R.C. Dhiman1
1National Institute of Malaria Research (ICMR), New Delhi; 2Primary Health Centre, Murumgaon, Gadchiroli district; 3District Malaria Office, Gadchiroli district, Maharashtra
Correspondence to: Dr R.K. Singh, Research Scientist, National Institute of Malaria Research (ICMR), Sector-8, Dwarka, New Delhi-110 077.
Received: 3 November 2011 Accepted in revised form:17 January 2012